34.4 Amino acids · Vary the pH, find the form and net charge of an amino acid or dipeptide, locate its isoelectric point and predict which way it moves on electrophoresis.
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About this lab
Vary the pH, find the form and net charge of an amino acid or dipeptide, locate its isoelectric point and predict which way it moves on electrophoresis.
You will be able to
Describe the acid/base properties of an amino acid and draw its cation, zwitterion and anion at a given pH.
Use pKa values and the pH to decide the form and net charge (an extension: the syllabus gives no pKa data), and find the isoelectric point.
Predict the direction and relative distance of migration of amino acids and a dipeptide on electrophoresis at a stated pH.
