Chromatogram Interpretation

What is Chromatogram Interpretation?

Reading a developed chromatogram to draw conclusions about a sample. An unknown is identified by running it alongside known substances on the same paper in the same solvent and matching the positions of the spots. The number of spots produced by a sample shows whether it is a single substance or a mixture: one spot is interpreted as pure and more than one spot as impure.

This definition is part of the Experimental Techniques and Chemical Analysis chapter in Cambridge O Level Chemistry.

Concepts related to Chromatogram Interpretation

• same conditions
• reference lane
• purity
• Retention Factor